廠牌: Immunochemistry 產地: 美國
Hoechst 33342 Fluorescent Nucleic Acid Stain
貨號：639
售價: 3918元
Size: 1 mL
Hoechst 33342是一種常用的細胞穿透性核染料,當它與雙鏈DNA結合時會發出藍色螢光。此染料常被用來區分凋亡細胞中凝聚的瓷樣細胞核,也被用於細胞週期研究。
這種染料可以穿透細胞膜進入細胞核內,與雙股螺旋DNA分子結合後,會產生強烈的藍色螢光發射。由於這種螢光強度與DNA含量成正比,因此Hoechst 33342被廣泛用於觀察細胞凋亡時細胞核的形態變化,以及分析細胞週期中DNA含量的變化。
它具有以下特點:
可以穿透活細胞膜,染色細胞核內DNA
發射藍色螢光,與其他綠色或紅色螢光蛋白質染料相容
螢光強度與細胞內DNA含量成正比
常用於偵測細胞凋亡、細胞週期分析等研究
總的來說,Hoechst 33342是一種極為實用的核酸染料,可應用於許多細胞生物學和分子生物學研究領域。
Background Hoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear morphology and for cell cycle studies.
Excitation / Emission 350 nm / 480 nm
Method of Analysis Flow cytometry, Fluorescence microscope
Storage 2-8°C
PH 5.0 + 0.5
Supplied At 200 µg/mL
Country of Origin United States
Protocols
Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 µL Hoechst to 300 µL of cells. 2. Incubate 10-20 minutes at room temperature.
Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.
Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.
When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.
Citations(31)
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