DRAQ7 遠紅活細胞未滲透 DNA 染料

DRAQ7™ 是一種活細胞不滲透、發射遠紅光的 DNA 染料，用於活力、樣品質量、細胞凋亡和固定細胞核複染。DRAQ7™ 具有許多細胞分析應用程序，並且與各種儀器平台上的現有協議高度相容。本產品可用於快速染色死細胞或透化細胞的 dsDNA/細胞核，並可與活細胞染料結合使用以區分活細胞/死細胞。

Background DRAQ7™ Far-Red Fluorescent DNA Dye is a membrane impermeant DNA dye for the rapid and convenient staining of the nuclear DNA of cells, tissues, and organisms including dead, permeabilized or fixed mammalian cells. It is ideal as a live/dead stain to assess sample quality, in apoptosis and cytotoxicity studies, and for immunofluorescence, immunohistochemistry, and high content screening applications. DRAQ7 far-red live-cell impermeant DNA dye differentially stains both the nucleus and cytoplasm in dead, permeabilized or fixed mammalian cells, making it ideal for a HCS translocation (or Redistribution™ / Transfluor™) assay. The intensity thresholding for the two compartments improves an in silico “dilation” while providing morphometrics. DRAQ7 directly and stoichiometrically reports DNA content in cells that are dead, permeabilized or fixed. Because DRAQ7 is a far-red emitting DNA dye, this can be easily multiplexed with other parameters, like cell surface phenotype.

Excitation / Emission 633 & 647 nm line optimal / 665 – 800 nm

Method of Analysis Flow cytometry, Fluorescence microscope

Sample Type Cell culture

Storage 2-8°C

Supplied At 0.3 mM

Country of Origin United States

Protocols

This product will provide 200 assay if using flow cytometry or 300 assays for fluorescent imaging. For additional protocol information, please visit www.biostatus.com