碘化丙啶染色劑

碘化丙啶 (PI) 是一種嵌入的螢光劑，可結合在 DNA 的鹼基之間。碘化丙啶是不透膜的，可防止 DNA 與活細胞結合，從而可以識別群體中的死細胞。

Background PI is an intercalating red fluorescent agent that binds between the bases of DNA. Upon binding to DNA, the fluorescence of Propidium Iodide (PI) is enhanced 20 to 30 fold. PI binding shifts the maximum fluorescence excitation by about 35 nm towards red while also shifting the maximum emission by about 15 nm towards blue. One molecule of PI stoichiometrically binds every four to five base pairs of DNA.

Target Necrotic and apoptotic cells

Excitation / Emission 535 nm / 617 nm

Method of Analysis Flow cytometry, Fluorescence microscope

Storage 2-8°C

PH 4.0 + 1.0

Supplied At 250 µg/mL

Country of Origin United States

Protocols

1. Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature.

2. Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm.

3. Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3.

4. When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.

Citations (2)

Fante, T;Simino, LAP;Fontana, MF;Reginato, A;Ramalheira, TG;Rodrigues, HG;Lisboa, PC;de Moura, EG;Ignácio-Souza, LM;Milanski, M;Torsoni, MA;Torsoni, AS. Maternal high-fat diet consumption programs male offspring to mitigate complications in liver regeneration. Journal of developmental origins of health and disease. 2021 December 3; doi: 10.1017/S2040174421000659. Full Abstract Niu, T;De Rosny, C;Chautard, S;Rey, A;Patoli, D;Groslambert, M;Cosson, C;Lagrange, B;Zhang, Z;Visvikis, O;Hacot, S;Hologne, M;Walker, O;Wong, J;Wang, P;Ricci, R;Henry, T;Boyer, L;Petrilli, V;Py, BF. NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly. Nature communications. 2021 October 6; doi: 10.1038/s41467-021-26142-w. Article