吖啶橙染色液

吖啶橙用於細胞生理學和細胞週期狀態的螢光顯微鏡和流式細胞術分析。這種可滲透細胞的細胞染料可與許多其他染色技術和螢光底物（包括 Magic Red 系列熒光蛋白酶底物）結合使用。吖啶橙是一種有用的細菌染色劑，可用於微生物的螢光顯微鏡檢查。

Background

Acridine Orange (AO) is a slightly cationic, lipophilic, fluorochrome stain capable of permeating cell and organelle membrane structure. Although quite cell permeant in the neutral form, once protonated, these dyes tend to become trapped on the low pH side of the membrane barrier leading to their accumulation in acidic organelle structures, such as lysosomes. Acridine Orange (AO), due to its metachromatic properties, is commonly used in fluorescence microscopy and flow cytometry analysis of cellular physiology and cell cycle status, including the fluorescent microscopic examination of microorganisms. Proton pump-driven lysosomal acidity generates a significant pH gradient resulting in the efficient concentration of AO within the lysosome organelles. The effectiveness of this AO concentration process is sufficient to create intra-lysosomal concentrations leading to precipitation of the AO into aggregated granules. These oligomeric structures exhibit a red shift (640 nm) compared to the monomeric AO that emits at 525 nm. Acridine Orange (AO) can be utilized in conjunction with a number of other staining techniques and fluorogenic substrates, including the Magic Red Cathepsin B substrate and the Magic Red Caspase 3/7 that detects caspase 3/7 activation in apoptotic cells.

Excitation / Emission

492 nm / 525 nm (monomeric form), 502 nm / 520-524 nm (Aggregated or DNA complexed form), 457 nm / 630-644 nm (Aggregated or RNA complexed form)

Method of Analysis

Flow cytometry, Fluorescence microscope

Storage

2-8°C

PH

5.0 + 0.5

Supplied At

1 mM, 266 µg/mL

Country of Origin

United States